The purpose of this study is to identify the barriers of application of precision farming in the view of agricultural researchers in Khouzestan province. The research method was survey. Agricultural researchers in Khouzestan province considered as statistical population (N=136). All researchers considered as sample size by census method. After designing and validating the questionnaire, the questionnaire was pre-tested among 30 people and Cronbach alpha 0.753 was determined. The main tool was a questionnaire. In this study, after the collection and classification of data, data analysis and according to the type of research in two stages using descriptive statistics and inferential statistics were taken. All data processing and statistical analysis was performed using the software SPSS 19. For analysis data, correlative coefficients and factor analysis were used. The results of the factor analysis indicate that lack of educational activity and managerial practices, lack of knowledge and information, lack of technical infrastructure, lack of support services, non-compliance with the conditions of farmers are five factors that explains about 57.65% of the variance barriers to the application of precision agriculture.