The purpose of this study was analyzing perception of agricultural college experts and managers of Islamic Azad University Shoushtar Branch regarding development of knowledge management. Agricultural college experts and managers were considered as a statistical population (N=38). This research was conducted in June 2012 to April 2013. All individuals were investigated. After confirm the validity of the instrument by panel of experts, to determine the reliability was used Cronbach alpha coefficient. Cronbach alpha coefficient was obtained for all sections of the questionnaire over 0.81. Method of research was descriptive and correlative. Based on the results, the correlation between status of knowledge acquisition and absorption with knowledge management in 0.01 level, was significant. Also the correlation between organizational culture and perceptions of experts and managers in 0.05 level, was significant. The results also showed that organizational culture, leadership style and knowledge about IT can explain 74% of variance of perception of agricultural college experts and managers regarding the development of knowledge management. [Masoud Ahmadinejad. Analyzing Knowledge Management of Experts and Managers in Agricultural College of Islamic Azad University, Shoushtar Branch. International Journal of Agricultural Science, Research and Technology in Extension and Education Systems, 2012; 2(4):171-174].