The purpose of this research was analyzing knowledge of rural cooperatives managers in Khouzestan province toward electronic commerce, Iran. The research method was correlative descriptive. The population of this study included rural cooperatives managers in Khuzestan province. The total number of members was 101 people. Due to the limited population, census method was used. Questionnaire reliability was estimated by calculating Cronbach’s alpha and it was appropriate for this study. There was between 0.771 to 0.842. Data were analyzed using the Statistical Package for the Social Sciences (SPSS). To reach the research objectives, appropriate statistical procedures for description were used. Data analysis was carried out through data description and data inferential analysis. Based on the results 65% of managers had low and very low knowledge toward electronic commerce in rural cooperatives. The results of research showed the correlation between level of education, creativity, technical requirements, social requirements, educational requirements, managerial requirements, economical requirements, political requirements with knowledge of electronic commerce in rural cooperatives was significant. Also the result of regression analysis by stepwise method indicated level of education, creativity, attitude to technical requirements, social requirements, educational requirements, managerial requirements, economical requirements and political requirements may well explain for 78.9% changes (R2 = 0.789) in knowledge of managers.