1 M.Sc. Graduate Department of Soil Science, Tabriz University, Iran

2 PhD student, Young Researchers Club’s Member, Islamic Azad University, Central Branch, Tehran, Iran

3 3Tehran Department of Environment, Iran


Plant biomass harvested after heavy-metal phytoremediation must be considered as a hazardous waste that should be contained or treated appropriately before disposal or reuse. This study provides an evaluation of extractants for the removal of lead from Abutilon Theophrastus biomass. The research was carried out a leaching study to determine the lead-extraction efficiency of the different leachants (water, several aqueous ammonium salts, and ethylenediaminetetraacetic acid solution as lead extractants). The result of this study showed that, lead concentrations measured in leached biomass and in leachates were significantly different among the various leachants. Also the extraction strength of the leachants followed the order ethylenediaminetetraacetic acid>ammonium citrate> water ammonium phosphate > ammonium acetate, to , achieving lead extraction efficiencies of 96%, 67%, 4.2%, 3.9% and 0.3%, respectively, in single-stage extractions. In this study, ethylenediaminetetraacetic acid is the most frequently lead extractants. (Akbarpour, F et al. Leaching of heavy metal from native plants by chemical leachants. International Journal of Agricultural Science, Research and Technology, 2011; 1(4):149-157).


1- Alkorta, I., Hern´andez-Allica, J., Becerril, J. M., Amezaga, I., Albizu, I and Garbisu , C. (2004). Recent findings on the phytoremediation of soils contaminated with environmentally toxic heavy metals and metalloids such as zinc, cadmium, lead and arsenic, Rev. Environ. Sci. Bio/Technol. 3, 71–90.
2- Baig, T. H., Garcia, A. E., Tiemann, K. J and Gardea-Torresdey, J. L. (1999). Adsorption of heavy metals ions by the biomass of Solanum elaeagnifolium (silverleaf night-shade), in: Proceeding of the 1999 Conference on Hazardous Waste Research, pp. 131–142.632
3- Baranowska-Morek, A., and Wierzbicka, M. (2004). Localization of lead in root tip of Dianthus carthusianorum, Acta Biol. Cracoviensia Ser. Bot. 46, 45–56.
4- Brooks, R. R., Anderson, C., Stewart, R. B and Robinson, B. H. (1999). Phytomining: growing a crop of a metal, Biologist 46, 201–205.
5- Dushenkov, S. (2003). Trends in phytoremediation of radionuclides, Plant Soil 249, 167–175.
6- Dushenkov, V., Kumar, P. B. A. N., Motto, H and Raskin I. (1995). Rhizofiltration: the use of plants to remove heavy metals from aqueous streams, Environ. Sci.Technol. 29, 1239–1245.
7- Elliot, H. A and Herzig, L .M. (1999). Oxalate extraction of Pb and Zn from polluted soils: solubility limitations, J. Soil Contam. 8 (1), 105–116.
8- Environmental Protection Agency. (EPA). USA 1998, Methods for Analytes and Properties, OSW Methods Team, United States Environmental Protection Agency.
9- Evangelou, M. W. H., Ebel, M and Schaeffer, A. (2007). Chelate assisted phytoextraction of heavy metals from soils. Effect, mechanism, toxicity, and fate of chelating agents. Chemosphere. 68, 989-1003.
10- Franceschi, V. R and Nakata, P. A. (2005). Calcium oxalate in plants: formation and function, Annu. Rev. Plant. Biol. 56, 41–71.
11- Gammons, C. H and Wood. S. A. (2000). The aqueous geochemistry of REE. Part 8: solubility of ytterbium oxalate and the stability of Yb (III)–oxalate complexes in water at 25 ◦C to 80◦C, Chem. Geol. 166, 103–124.
12- Ghosh, M and Singh, S. P. (2005). A review on phytoremediation of heavy metals and utilization of its byproducts, Appl. Ecol. Environ. Res. 3, 1–18.
13- Gramss, G., Voigt, K. D and Bergmann, H. (2004). Plant availability and leaching of (heavy) metals from ammonium-, calcium-, carbohydrate-, and citric acid-treated uranium-mine-dump soil, J. Plant Nutr. Soil Sci. 167, 417–427.
14- Keller, C., Ludwig, C., Davoli, F and Wochele, J. (2005). Thermal treatment of metal enriched biomass produced from heavy metal phytoextraction, Environ.Sci.Technol.39, 3359–3367.
15-Kim, C and Ong, S. K. (1999). Recycling of lead-contaminated EDTA wastewater, J.Hazard. Mater. B69, 273–286.
16- Kos, B and Leˇstan, D. (2003). Phytoextraction of lead, zinc and cadmium soil by selected plants, Plant Soil Environ. 49, 548–553.
17- Luo, C. L., Shen, Z. G., Li, X. D. ( 2005). Enhanced phytoextraction of Cu, Pb, Zn and Cd with EDTA and EDDS. Chemosphere. 59, 1-11.
18- Luo, C. L., Shen, Z. G., Lou, L. Q., Li, X. D. (2006). EDDS and EDTA-enhanced phytoextraction of metals from artificially contaminated soil and residual effects of chelant compounds. Environmental Pollution .144, 862-871.
19- Luo, C. L., Shen, Z. G., Li, X. D. (2007). Plant uptake and the leaching of metals during the hot EDDS-enhanced phytoextraction process. International Journal of Phytoremediation .9, 181-196.
20- Lytle C. M., Lytle, F.W., Yang Qian, N. J., Hansen, H. D., Sayed, A and Terry, N. (1998). Reduction of Cr(VI) to Cr(III) by wetland plants; potential for in situ metal etoxification, Environ. Sci. Technol. 32, 3087–3093.
21- Macek, T., Mackov´a, M., and K´as, J. (2000). Exploitation of plants for the removal of organics in environmental remediation, Biotechnol. Adv. 18, 23–34.
22- Mazen, A. M. A and El Maghraby, O. (1997). Accumulation of cadmium, lead and strontium, and a role of calcium oxalate in water hyacinth tolerance, Biol.Planta. 40 (3): 411–417.
23- Mazen, A. M. A., Zhang D and Franceschi, V. R. (2003). Calcium oxalate formation in Lemna minor: physiological and ultraestructural aspects of high capacity calcium sequestration, New Phytol. 161, 435–448.
24- Nowack, B., Schulin, R and Robinson, B. H. (2006). A critical assessment of chelantenhancedmetal phytoextraction, Environ. Sci. Technol. 40 (17): 5525–5532.
25- N´u˜nez-L´opez, A. R., Meas, Y., Gama, S. C., Borges, R. O and Olgu´ın, E. J. (2008). Leaching of lead by ammonium salts and EDTA from Salvinia minima biomass produced during aquatic phytoremediation. Journal Hazardous Material, 154: 623-632.
26-Olgu´ın, E. J., Anchez-Galv´an. G. S and Erez-P´erez, T. P. (2005). Surface adsorption, intracellular accumulation, and compartmentalization of Pb (II) in batch-operated lagoons with Salvinia minima as affected by environmental conditions, EDTA and nutrients, J. Ind. Microbiol. Biotechnol. 32: 577–586.
27- Peters, R.W. (1999). Chelant extraction of heavy metals from contaminated soils. Hazard. Mater. 66, 151–210.
28- Polprasert, Ch. (1996). Organic Waste Recycling, second ed., John Wiley & ons, Chichester, UK.
29- Quevauviller, P., Rauret, G and Griepink, B. (1993). Single and sequential extraction in sediments and soils, conclusions of the workshop, Int. J. Environ. Anal.Chem. 51, 231–235.
30- Rathinasabapathi, B., Ma, L. Q and Srivastava, M. (2006). Arsenic hyperaccumulating ferns and their application to phytoremediation of arsenic contaminated sites, in: J.A. Texeira da Silva (Ed.), Floriculture, Ornamental and Plant Biotechnology, Global Science Books, London, pp. 304–311.
31- Reed, S.C.; Crites, R.W.; and Middlebrooks, E.J. (1995). Natural Systems for Waste Management and Treatment, second ed., McGraw-Hill, NY.
32-Robinson, B. H., Mills, T. M., Petit, D., Fung, L. E. Green, S. R and Clothier, B. E. (2000). Natural and induced cadmium-accumulation in poplar and willow: implications for phytoremediation, Plant Soil 227, 301–306.
33- Römkens, P., Bouwman, L., Japenga, J and Draaisma, C. (2002). Potential drawbacks of chelat- enhanced phytoremediation of soils. Environmental Pollution.116, 109-121.
34- Sas-Nowosielska, A., Kucharski, R., Małkowski, E., Pogrzeba, M., Kuperberg, J. Mand Kry´nski, K. (2004). Phytoextraction crop disposal—an unsolved problem, Environ. Pollut. 128, 373–379.
35- Sun, B., Zhao, F.J. Lombi, E and McGrath, S. P. (2001). Leaching of heavy metals from contaminated soils using EDTA, Environ. Pollut. 113, 111–120.
36- Tandy, S., Bossart, K., Mueller, R., Ritschel, J., Hauser, L., Schulin, R., and Nowack, B. (2004). Extraction of heavy metals from soils using biodegradable chelating agents, Environ. Sci. Technol. 38 (3), 937–944.
37- Yang, Y. Y., Jung, J. Y., Song, W.Y., Suh, H. S and Lee, Y. (2000). Identification of rice varieties with high tolerance or sensitivity to lead and characterization of the mechanism of tolerance, Plant Physiol. 124, 1019–1026.
38- Zeng, Q. R., Sauv´e, S., Allen, H. E and Hendershot, W. H. (2005). Recycling EDTA solutions used to remediate metal-polluted soils, Environ. Pollut. 133, 225–231.